STONY BROOK, NY, November 12, 2021 – The genomic revolution has enabled researchers to evaluate cell-by-cell genetic variations, however only a few methods exist to measure cell-by-cell metabolic variations, a extra highly effective approach to perceive cell responses to altering environmental situations. Researchers from Stony Brook College’s College of Marine and Atmospheric Sciences (SoMAS), led by Gordon T. Taylor, PhD, demonstrated that Raman microspectroscopy can precisely measure cell-by-cell variations in progress charges of the bacterium E. coli grown in a broth medium. They validated the Raman-based method in opposition to unbiased conventional population-based spectroscopic and mass spectrometric measurements.
“The method rising from our laboratory might be utilized to the examine of free-living and host-associated microbiomes, which may show essential in understanding extra about their purposeful responses to stressors,” says Taylor, Professor and Director of the NAno Raman Molecular Laboratory (NARMIL) at SoMAS. “We additionally imagine that is an enabling expertise to look at individuality in cell populations and will have broad functions in microbiology, cell biology and biomedicine.”
Particulars of the method and outcomes are revealed within the American Society of Microbiology’s Applied and Environmental Microbiology. A visible of the method can be highlighted on the duvet of the journal version.